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Zooplankton were collected in spring and late summer/fall at four stations representing the salinity gradient in the Parker River-Plum Island Sound estuary. Two size classes, greater than 335 micron and greater than 150 micron, were collected by net tows. Conductivity or salinty and temperature were recorded for each sample. Samples were concentrated to less than 250 mls and preserved in 70 percent EtOH. For taxonomy, sample splits were taken such that a minimum of 250 individuals were present, and counted under a dissecting microscope.